Antibacterial Properties of Tchihatchewia isatidea Boiss. from Turkey

  • Kenan TUNÇ
  • Ayşegül HOŞ
  • Erdal KAYA
Keywords: Antibacterial Activity, Disc Diffusion Method, Tchihatchewia isatidea Boiss.

Abstract

Development of antimicrobial agents obtained from plant extract is among the noteworthy topics to resolve the bacterial problems
associated with environment, food safety, animal and human health. In this study, antibacterial activity of methanolic extract of
Tchihatchewia isatidea Boiss. (crude extract) was determined. Dried powder of the parts (flower, leave and stem) of Tchihatchewia isatidea
Boiss. were kept in methanol for 3 days in dark place. The antibacterial activity of crude extract was determined by disc diffusion method.
Methanol-impregnated discs were used as negative control. Also commercial antibiotic discs (Clindamycin (10 µg), Tetracycline (10 µg) and
Amoxicillin-Clavulanic acid (30 µg)) were utilized as positive control. According to the experimental results of this study, the methanolic
extract of Tchihatchewia isatidea Boiss. (At the doses of 800 µg/10µl, 400 µg/10µl, 200 µg/10µl and 100 µg/10µl) have been found to have
antibacterial activity against Staphylococcus aureus ATCC 29213. It was determined that methanolic extract of Tchihatchewia isatidea
Boiss. has showed no antibacterial activity against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Enterococcus faecalis
ATCC 29212, Salmonella typhimurium ATCC 14028, Streptococcus mutans CNCTC 8/77, Streptococcus mitis CNCTC 4/77, Streptococcus
salivarius CNCTC 64/59 and Staphylococcus epidermidis ATCC 12228. The antibacterial activity of methanolic extract of Tchihatchewia
isatidea Boiss. was investigated in this study for the first time.

Published
2019-07-08
Section
Articles