PHYLOGENETIC DIVERSITY OF NOCARDIA SP. OBTAINED FROM DIFFERENT WATER ENVIRONMENTS

  • Şükrü Önalan Van Yüzüncü Yıl University
  • Hamdullah Seçkin Van Yüzüncü Yıl University
Keywords: Nocardia sp., Real-Time PCR, Sequencing, Phylogenetic diversity

Abstract

The aim of this study was to investigate the isolation and typing of Nocardia species, which are found in many water resources and have microbial importance. Agent isolations were carried out from sediment samples taken from different points determined around Iğdır Fish Lake. DNA isolations were carried out from the isolated Nocardia species. Subsequently, 16S rRNA gene region sequence analyzes were performed. Sequence analyzes for each isolated bacteria were aligned and these results determined similarities and differences at the level of group and genus of different origins and individual populations. As a result of the study, 2 different types of bacteria were isolated. In the GenBank database, the isolated bacteria were found to have 99.78% and 99.30% similarity with the species with 99% and higher sequence matching. Nucleotide sequencing differences were found to be 0.3% with reported to be isolated from different water sources. Different studies are needed to determine the differences at the gene level of the same type of bacteria isolated from different sources, and to determine the pathogenicity and virulence characteristics among the isolates.

Published
2020-12-17
Section
Articles