İpekböceği (Bombycidae: Bombyx Mori)’nin Farklı Genomik İçerikli Yumurtalarında Vitellin Proteini Özelliklerinin Araştırılması

  • Gamze TURGAY İZZETOĞLU
  • Beria FALAKALI MUTAF
Keywords: Silkworm, egg, polyacrylamide gel electrophoresis (PAGE), vitellin.

Abstract

The eggs of silkworm (Bombyx mori), are naturally diploid or haploid and, are easily inducible into polyploidy. The present work deals with the
comparison of yolk protein, vitellin, of the eggs with different genomic contents. The eggs obtained from Silkworm Research Institute of Bursa (BIAE)
manipulated to make them differ in genome size and physiological conditions, were homogenized to measure total amount of proteins and especially to scan
their vitellin phenotypes by means of PAGE. There were two bands of vitellin standart solution but there observed no eminent differences on the samples.
Only slight variations on the density of second band of vitellin were postulated as a reference to the utilization of these yolk proteins during the embryonic
development. Those small dispairments of the phenotypes, although reckoning with the large array of manipulations, suggest that vitellin reaches stability in
amount after uptake into the egg. So, similar survey should be carried on much earlier, during vitellogenesis.

Published
2019-07-04
Section
Articles