Patlıcan Kallus Kültüründe Fitoaleksin Uyartımı Üzerinde Araşidonik Asit Elisitörünün Etkisi

Özet

Wild species Solanum sisymbriifolium has a high resistance against many biotic and abiotic stress factors including Verticillium dahliae resistance, with respect to S.melongena that is culture form of eggplant. In this study we aimed to obtain some information about the mutual influence of " phytoalexin accumulation - resistance to V.dahliae" by using genotypes which are resistant (S. sisymbriifolium) or susceptible (Long Purple eggplant variety) to V.dahliae and in the period of time after the elicitation treatment and two different dosages of arachidonic acid elicitor. In order to obtain callus from hypocotyle explants of the in vitro eggplant seedlings, 0.5 mg L-12,4-D ve 0.1 mg L-1 kinetin added to the MS medium was used. 5 and 10 mM arachidonic acid were used as elicitor. 24, 48 ve 72 hours were used as elicitation time. Thin-layer chromotography technique was used to obtain solavetivone, lubimin and rishitin phytoalexins standarts by using the elicited potato tubers. The extracted substances were analyzed with GLC. Solavetivone phytoalexin accumulation was determined in the callus cultures
of the eggplant genotypes. The highest accumulation was obtained in “10 mM arachidonic acid x 72 hours elicitation” application. The interactions of "cultivar x elicitor concentration x elicitation time" were effective on the solavetivone accumulation. The elicitor doses used in this study was insufficient for phytoalexin accumulation in resistance species under callus culture conditions. However, at the end of the experiments we made with in vitro conditions it was concluded that it would not be possible to establish a reliable and definite relationship about the resistance of different genotypes to V.dahliae,
by determining the phytoalexin accumulation.