Patlıcanda Kotiledon Eksplantından Organogenezis Sağlanması Üzerinde Çalışmalar

Özet

Vegetatif propagation of plant materials obtained in vitro conditions or in a limited number can be made
important. In this study, we aimed to determinate the composition of aculture medium suitable for in vitro plant
regeneration of eggplants also to investigate the effect of genotypes to regeneration capacity. As plant material
two open pollinated eggplant varieties (Long Purple and Topan), one local hybrid genotype (3x8) and a wild
species (Solanum integrifolium) was used. Cotyledon pieces of seedlings were used as explants and that
seedlings were obtained from eggplant seeds which are germinated in vitro conditions. The composition of the
basic MS medium was used and this medium was enriched with two different carbohydrate sources (glucose,
1.5% or 2% sucrose). "2 mg/l zeatin and 0.01 mg/l NAA," "4 mg/l NAA," "10 mg/l NAA", are added as growth
regulators. Experiment was established according to randomized block experimental design. 50 cotyledon piece
explants were cultured for each application. The cultures were placed in a climate room in the darkness for 3
weeks and then photoperiodic system was applied. After one month observations made in cultures, callus
formation rate, presence of organogenesis (shoot and root formation) was determined and the amounts per
explant was detected. Different response among genotypes was observed on to effect of culture medium
composition. For the formation of shoots, it could be necessary to transfer the callus tissues into the new media
compositions including cytokinins. It is required the further investigations about the optimization of this method.