Androgenic Responses of Different Pepper (Capsicum annuum L.) Cultivars

Abstract

Factors such as genotype, donor plant, pre-treatments of buds or anthers, microspore development stage and incubation conditions affect
androgenetic response. Eleven different pepper types (4 longy, 1 paprika, 2 bell pepper, 2 capya, 2 long green) were used in this study with the aim
of reveal the genotypic response to anther culture. Flower buds excised when the corolla was of the same length as the calyx or slightly longer.
Flowers were kept in cold (+ 4oC) and in dark for 24 h. Murashige-Skoog medium with 8 g/l agar and 30g/l sucrose was used. Incubation conditions
were 35 oC for 8 days then transferred to 25 oC with 16/8 h dark-light period with 3000 lux light intensity. Results revealed that pepper types had
different androgenetic responses. A total of 44 embryos were derived from 2398 in vitro cultured anthers of 11 genotypes and 12 plantlets were
obtained. Yalova Carliston (long green type) and Kandil (bell type) did not produce any embryos. Sera Demre 8 (longy type) and Odesa (bell type)
gave the better androgenic response compare to other pepper genotypes.