Fusarium Türlerinin İzolasyonu ve Patojenitelerinin Belirlenmesi

Özet

The subject of our study was to define the pathogens by isolating in a laboratory environment in order to combat the Fusarium spp. which is
found in the soil and is an important plant pathogen that gives grate loss to the countries economy with the damage it causes.
The F. culmorum and F.graminearum are chosen as material which causes paleness sickness and root corrosion in lots of plants. The wheat
fields of Ankara, Eskisehir and Sakarya were chosen as workspace and the wheat that shows root and root neck corrosion were transferred to our
Laboratory and segments were taken from the plants and were made a cursory disinfection with %2 sodium hypochlorite and inoculated in Potato
Dextrose Agar and specific agar environments. They were incubated under close UV light (>256<400 nm) at 24±2°C for 1 week and the developing
funguses were investigated and were grouped according to their species. The pathogenicity tests were applied to the isolates chosen from these
species. The pathogenicity tests conducted both in Petri dishes and in flowerpot trials. Patojenite testleri hem petrilerde hem de saksı denemeleri
ile gerçekleştirilmiştir. The isolates that were chosen for this purpose were conducted an pre elimination test for pathogenicity in 250 µg/ml
chloramphenicol added Tap Water Agar on a sprouted wheat seed. The formed indicators were graded and the isolates that were sighted to have the
high degree affect were separated for the pathogenicity tests that will be conducted at the flowerpots. In the flowerpot trials the plant growths were
followed regularly and with the results at the end of 1 month the plants in the pots were registered as healthy and diseased. The highest pathogenitic
isolates were defined by determining the growing percentage and liveliness percentage.