Drechslera sorokiniana (Sacc)’nın İzolasyonu Ve Patojenitesinin Belirlenmesi

Özet

The pathogenicity and isolation of Drechslera sorokiniana was defined which causes grain mortality in the cereal fields and also composes
seedling burns, root and root neck burns, necrotic flecks, glumes and grain burns then specifically constitutes doted leaf fleck disease. The wheat
those were grown in the trail plots of Trakya Agricultural Research Institute which composed root and root neck corrosions were taken to the
laboratories. The blotting papers were placed inside the Petri dishes and were humidified according to Blotter method. Segments were taken
from the plants which their roots are blackened and were made a cursory disinfection with 2% sodium hypochlorite. The roots were then cut into
small pieces and were placed in to the humidified Petri dishes. They were incubated for 1 week and spores are procured. The formed konidies
were taken with sharp nosed transparent tapes and inoculated inside Potato Dextrose agar and the colonization formation was investigated. The
colonization period is relatively slow, blackish color, in the telescopic concentric circles like velvet. The pathogenicity tests conducted both in
Petri dishes and in flowerpot trials. The isolates that were chosen for this purpose were conducted an pre elimination test for pathogenicity in 250
µg/ml chloramphenicol added Tap Water Agar, (TWA) on a sprouted wheat seed. The formed indicators were graded and the isolates that were
sighted to have the high degree affect were separated for the pathogenicity tests that will be conducted at the flowerpots. In the flowerpot trials the
plant growths were followed regularly and with the results at 1 month the plants in the pots were registered as healthy and diseased. The highest
pathogenitic isolates were defined by determining the growing percentage and liveliness percentage.