Türkiye’de Bulunan Bazı Yerli Sığır Irklarının STR Markörler ile Genetik Karakterizasyonu

Özet

Changing environmental conditions have affecting the world’s fauna and flora irreversibly. Objective was genetic characterization and
determination of phylogenetic relationship of native cattle breeds in Turkey using microsatellite DNA markers as part of a national project titled
“In vitro Conservation and Preliminary Molecular Identification of Some Turkish Domestic Animal Genetic Resources-I (TURKHAYGEN-I)”.
Blood samples were collected from Anatolian Black (YK), Anatolian Grey (BI), South Anatolian Red (GAK), Native Southern Anatolian Yellow
(YGS), East Anatolian Red (DAK) and Zavot (ZAV) cattle. DNA samples were isolated using a standard phenol/chloroform method. A total of
20 microsatellite loci were selected from a list suggested by FAO and International Society of Genetics (ISAG). PCR products were separated by
capiller electrophoresis on Beckman Coulter CEQ-8000 Genetic Analysis System, and marker genotypes were determined. In statistical analyses,
numbers of alleles, deviation from Hardy-Weingberg Equilibrium parameters were calculated and phylogenetic trees were drawn. A total of 274
alleles were observed. The lowest allele number (8) was observed at INRA005 and BM1824 loci and the highest allele number (23) was calculated
for TGLA122. YGS, YK, GAK and DAK populations were closely localized than BI and ZAV in factorial correspondence analysis. The resulting
genetic parameters and phylogenetic relationship of breeds analyzed are consistent with their today’s geographical locations.