An Investigation of the Cytotoxic, INVESTIGATION OF THE CYTOTOXIC, GENOTOXIC AND PHYSIOLOGICAL EFFECTS OF INSECTICIDE KORBAN 25 W WITH THE ALLIUM ASSAYand Physiological Effects of insecticide Korban 25 W with the Allium assay

INVESTIGATION OF THE CYTOTOXIC, GENOTOXIC AND PHYSIOLOGICAL EFFECTS OF INSECTICIDE KORBAN 25 W WITH THE ALLIUM ASSAY

Abstract

Cytotoxic and physiological effects of the insecticide Korban 25 W [25% Chloropyrifos-Ethyl (0.0-diethyl 0-(3,5,6 trichloro 2-pyridyl) phosphorothrioate)] were investigated on Allium cepa L. The roots of the 5 days old seedlings were treated with 0 g/lt (control=tap water), 2 g/lt, 3 g/lt and 4 g/lt concentrations of Korban 25 W during 5 and 10 days.

Korban 25 W significantly decreased Mitotic Index (MI) and increased the chromosome aberrations (micronucleus, chromosome bridge, sticky metaphase) at all concentrations and treatment periods when compared with their controls. It was also determined that Korban 25 W enhanced the Malondialdehide (MDA) level by increasing the lipid peroxidation and caused changes in Superoxide dismutase (SOD) and Catalase (CAT) activities. All these changes were depending on dose and duration of applied insecticide.