Salvia ceratophylla L. and Ricotia aucheri (BOISS.) B.L. INVESTIGATION OF BIOLOGICAL AND CYTOTOXIC ACTIVITIES OF BURTT PLANTS IN VITRO CONDITIONS

Abstract

In this study, was aimed to investigate the antimicrobial activities, antioxidant capacity assays and cytotoxic effects of Salvia ceratophylla L. and Ricotia aucheri (Boiss.) B.L. Burtt on MCF-7 (human breast cancer cell line) and HUVEC (human umbilical vein endothelial cells) cell lines. Disc diffusion method was chosen for antimicrobial activity and capacity and it was determinedon 6 pathogenic microorganisms (Enterococcus durans, Staphylococcus aureus ATCC 25923, Enterobacter aerogenes ATCC 13048, Bacillus subtilis DSMZ 1971, Salmonella typhimurium). Antioxidant activity capacities were observed by DPPH method. Cytotoxicity tests on MCF-7 (human breast cancer cell line) and HUVEC (human umbilical vein endothelial cells) cell lines were tested by MTS method. While the antimicrobial effect of Ricotia aucheri was observed on Klebsiella pneumoniae and Staphylococcus aureus ATCC 25923 strains, antimicrobial effect of Salvia ceratophylla was observed on with other strains except Enterococcus durans and Enterobacter aerogenes ATCC 13048. It was also observed that Salvia ceratophylla, showed a higher antioxidant capacity than BHT when BHT was used as the control group. On the other hand, in the cytotoxicity test, Ricotia aucheri plant occurred a significant decrease in MCF-7 cell line. As a result of this study, it has been determined that two plant materials have antimicrobial, antioxidant and cytotoxic properties and it can be assumed that they can be a source for future studies.